Fig. 3
Porous Se@SiO2 nanospheres inhibit diabetes-induced retinal vasculopathy. A Representative images of the acellular capillaries (arrows) in trypsin-digested retinas. Scale bar, 40 μm. B Quantification of acellular capillaries per mm2 of retinal area (n = 8). C Quantification of Evans blue dye exudation from retinal vessels (n = 6). D Expression levels of ZO-1, occludin, and claudin-5 in retinas were measured using western blotting; β‐actin was used as a loading control. E Band densities were assessed using the ImageJ software, and the ZO-1 (left panel), occludin (middle panel), and claudin-5 (right panel) expression levels are represented as their ratios to β‐actin (n = 3). Data are represented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant