Fig. 5
Porous Se@SiO2 nanospheres mitigate HG-induced dysfunction of HRMECs via GPX4. A Representative images of HRMEC migration at 0 h (top panel) and 12 h (bottom panel). Scale bar, 50 μm. B Quantification of HRMEC migration rate (n = 3). C Representative images of HRMEC tube formation. Scale bar, 50 μm. Quantification of HRMEC tube lengths (D) and branch points (E) (n = 6). F Expression levels of ZO-1, occludin, and claudin-5 in HRMECs were measured using western blotting; β‐actin was used as a loading control. G The band densities were assessed with ImageJ software, and the ZO-1 (left panel), occludin (middle panel), and claudin-5 (right panel) expression levels are represented as their ratios to β‐actin (n = 3). Data are represented as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant