Fig. 1
Effects of CLP and CLP + xanomeline treatment on activity in the basal forebrain cholinergic system. C57Bl6 mice, euthanized 48 h. post CLP. A Representative immunostained sagittal sections. Scanned and imaged on Leica DMI 4000b using Allen Brain Atlas (P56 Image 21) at 20 × magnification. Green stain (fluorescence from Alexa 488, identified by blue arrows)—Choline Acetyl Transferase (ChAT)—containing neurons; Red stain (fluorescence from Alexa 594, indicated by yellow arrows)—c-Fos expressing neurons; neurons expressing both ChAT and c-Fos indicated by white arrows. B Quantification of active neurons that expressed ChAT. Immunofluorescent activity of ChAT (Alexa 488) and c-Fos (Alexa 594) determined in 10 non-contiguous 20x-powered fields per slide, 1–2 slides/animal. Mean value for each animal indicated by closed circle (T0/baseline), closed square (48 h. post CLP) or closed diamond (xanomeline treatment of mice studied 48 h. post-CLP). N = 4 mice for each set of measurements. Long horizontal line—mean of measurements in all four animals; lighter lines—± standard deviation. Significance determined using one-way ANOVA with Tukey–Kramer correction, P < 0.05. * = significantly different from T0. ^= significantly different from value at 48 h. post-CLP without xanomeline