Fig. 3
Effects of CLP and CLP + xanomeline treatment on serum concentrations of selected cytokines. C57Bl6 mice, euthanized 48 h. post CLP. Levels (pg/ml) determined using multiplex ELISA (Eve Technologies, Calgary, Alberta, Canada). Mean value for each animal indicated by closed circle (T0/baseline), closed square (48 h. post-CLP) or closed diamond (xanomeline treatment of mice studied 48 h. post-CLP). Linear Y-axis in IL-1b plot, log2 Y-axes in all other plots. Long horizontal lines—mean value (arithmetic for IL-1β, geometric for others); error bars—± standard deviation (arithmetic for IL-1β, geometric for others). For TNFα, MIP-1α, IL-6, KC, and G-CSF, data were ln-transformed for analysis. Statistical comparisons between groups with ordinary one-way ANOVA with Tukey–Kramer correction (P < 0.05) applied to results. *= significantly different from value at T0; ^= significantly different from value at 48 h. post-CLP without xanomeline. A Serum concentrations of TNFα, IL-1β and MIP-1α. N = 4–7. B Serum concentrations of IL-6, KC and G-CSF. N = 4–7