Fig. 4
Effects of CLP and CLP + xanomeline treatment on splenic cell subgroups. C57Bl6 mice, euthanized 48 h. post CLP. Cell types/cytokines identified using flow cytometry. Mean value for each animal indicated by closed circle (T0/baseline), closed square (48 h. post CLP) or closed diamond (xanomeline treatment of mice studied 48 Hrs. post-CLP). Long horizontal lines—mean value; error bars—± standard deviation. Significance determined using one-way ANOVA with Tukey–Kramer correction, P < 0.05. *= significantly different from value at T0; ^= significantly different from value at 48 h. post-CLP without xanomeline. N = 3–7. A Numbers of Neutrophils, Macrophages and Inflammatory Monocytes. B Numbers of Central DCs, CD4+ T cells and CD8+ T cells