Fig. 5
Effects of CLP and CLP + xanomeline treatment on innate immune splenic cells expression of TNFα and/or IL-1β. C57Bl6 mice, euthanized 48 h. post CLP. Cell types/cytokines identified using flow cytometry. Mean value for each animal indicated by closed circle (T0/baseline), closed square (48 h. post CLP) or closed diamond (xanomeline treatment of mice studied 48 Hrs. post-CLP). Long horizontal lines—mean value; error bars—± standard deviation. Significance determined using one-way ANOVA with Tukey–Kramer correction, P < 0.05. *= significantly different from value at T0; ^ = significantly different from value at 48 h. post-CLP without xanomeline. N = 3–7. A Numbers of Neutrophils and Monocytes that expressed TNFα or IL-1β in response to ex vivo stimulation with LPS. B Percentages of Neutrophils and Monocytes that expressed TNFα or IL-1β in response to ex vivo stimulation with LPS