Fig. 2
From: Extracellular CIRP induces CD4CD8αα intraepithelial lymphocyte cytotoxicity in sepsis
CIRP deficiency attenuates CD4CD8αα IEL cytotoxicity in septic mice. WT and CIRP−/− mice were subjected to sham or CLP and small intestine samples were collected 4 h after the surgery to assess cytotoxic granule levels of CD4CD8αα IELs using flow cytometry. (A, D) PercentageRepresentative histogram, (B, E) MFIpercentage and (C, F) histogramMFI of (A-C) GrB and (D-F) Prf expressions in CD4CD8αα IELs are shown. Experiments were performed 3 times, and all data were used for analysis. Data represent the mean ± SEM (n = 7–9/group). The groups were compared by one-way ANOVA followed by a Tukey’s multiple comparisons test t. *p < 0.05 vs. WT sham, #p < 0.05 vs. WT CLP.