Fig. 3
From: Extracellular CIRP induces CD4CD8αα intraepithelial lymphocyte cytotoxicity in sepsis
eCIRP enhances cytotoxicity in CD4CD8αα IELs via TLR4. IELs isolated from WT or TLR4−/− mice were treated with PBS or eCIRP (5 μg/ml) for 3 h and cytotoxic granule levels of CD4CD8αα IELs were assessed by flow cytometry. (A, D) Representative histograms, (B, E) percentage and (C, F) MFI of (A-C) GrB and (D-F) Prf in CD4CD8αα IELs are shown. Experiments were performed 2 times, and all data were used for analysis. Data represent the mean ± SEM (n = 5/group). The groups were compared by one-way ANOVA followed by a Tukey’s multiple comparisons test. *p < 0.05 vs. WT PBS, #p < 0.05 vs. WT eCIRP.