Fig. 5

CIRP deficiency attenuates injury and cell death in intestinal epithelium of septic mice. Small intestines of WT or CIRP^−/− mice subjected to sham or CLP surgery were harvested for histological analysis. (A) Representative H&E images are shown at ×200 original magnification. Scale bars; 50 μm. (B) Villous height was measured. (C) Representative immunofluorescence images of TUNEL (green) staining and nuclear counterstaining (blue) are shown at ×200 original magnification. Scale bars; 50 μm. (D) Numbers of TUNEL⁺ cells in small intestinal tissues. Experiments were performed 2 times, and all data were used for analysis. A random selection of 3 high power fields (×200) were analyzed per mouse. Data represent the mean ± SEM (n = 5–10/ group). Scale bars; 50 μm. The groups were compared by one-way ANOVA followed by a Tukey’s multiple comparisons test. *p < 0.05 vs. WT sham, ^#p < 0.05 vs. WT CLP.