Fig. 2

Fluvoxamine inhibits the activation of CD4⁺ T cells but does not affect dendritic cells, macrophages, and CD8⁺ T cells in the pancreatic lymph nodes (PLNs). PLN cells from 12-week-old fluvoxamine-treated and vehicle-treated mice were harvested and subject to flow cytometry analysis. Representative FACS plots of A DCs (MHCII⁺CD11C⁺) and B analysis of the MFI of CD80 and CD86. Representative FACS plots of C macrophages (CD11b⁺F4/80⁺) and D analysis of the MFI of CD80 and CD86. The frequencies of E CD4⁺, F CD8⁺, G CD4⁺CD44^highCD62L^lo (CD4 effector T cells) and H CD8⁺CD44^highCD62L^lo (CD8 effector T cells) are shown (n = 5 per group). Data are expressed as mean ± SEM. Statistical significance was calculated by unpaired Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001, and ns, not significant