Fig. 1

BATF expression is positively correlated with effector CD8⁺ T cell differentiation. A Splenocytes from WT B6 mice were stimulated with 2 µg/ml soluble anti-CD3e mAb and 1µg/ml soluble CD28 for 24 h, followed by RNA- seq analysis and flow cytometric analysis. One WT B6 mice were used for isolated CD8+ T cells and stimulation of CD8+ T cells. B The volcano plot shows up- and down- regulated genes in 0h stimulated CD8+ T cells and 24h stimulated CD8+ T cells (red dots). The STAR program was used to align the reads to the mouse reference file (GENCODE mouse reference GRCm38). R package (edgeR) was used to analyze differential expression with raw read count of each gene adjusted by trimmed mean of M values (TMM). The significant differentially expressed genes are both padjust < 0.05 and |log2(FoldChange)|> 1. C Heatmap based on FPKM of selected differentially expressed genes. Color is proportional to the relative abundance of gene expression cross sample, that is blue stands for low expression and red stands for high expression. D The graph shows Batf MFI of CD8+ T cells. E, F Representative contour plots and bar graphs show the expression of IFN-γ and granzyme B in CD8+ T-cell subpopulations that express high, medium or low levels of batf. G Counts and MFI in three batf repressed CD8+ T-cell subpopulations. Data are mean ± SD (n = 5). **p < 0.01, ****p < 0.0001 (unpaired Student’s t-test)