Fig. 5
From: BATF and BATF3 deficiency alters CD8+ effector/exhausted T cells balance in skin transplantation
BATF and BATF3 deficiency attenuates early functional expression of effector CD8+ T cells. A B6.Rag1-/-recipients were adoptively transferred with either batf−/− batf3−/− or control CD8+ T cells, followed by BALB/c skin transplantation. Adoptively transferred CD8+ T cells in spleens were analysed by flow cytometry at day 14 post-skin grafting. A, B Representative contour plots and bar graphs show % of IFN-γ+ and IFN-γ+IL-2+CD8+ T cells in spleen CD8+ T cells. C–F Contour plots were gated on the living transferred CD8+ T cells in spleens. Representative contour plots and bar graphs show % GZB+CD8+ (C, D) and Ki67+CD8+ (E, G) cells in spleen CD8+ T cells. *, p < 0.05; **, p < 0.01. G, H Representative fluorescence intensity distribution maps and MFI bar graphs of IRF4, T-bet and Id2 in spleen CD8 + T cells. **p < 0.01 by unpaired Student’s t-test