Fig. 2

PDGF signaling mediates chronic inflammation in the hypothalamus. A Representative confocal images of cFos (green) and POMC (red) immunoreactivities and counterstaining with Hoechst 33342 (blue) in the ARC of FL and Pdgfrb^∆SYS-KO fed HFD for 4 weeks (n = 7–8). Scale bar = 200 μm. The right two panels are high magnification of the gray line square area. White arrows indicate Hoechst⁺cFos⁺POMC⁺ cells. Scale bar = 50 or 20 μm. The ratio of POMC-positive neurons in Hoechst-positive cells and cFos-POMC double-positive neurons in POMC-positive neurons are quantified. B Representative confocal images of UCP1 immunoreactivity (green) and Hoechst 33,342 (blue) staining in the BAT of FL and Pdgfrb^∆SYS-KO fed HFD for 4 weeks. Scale bar = 100 μm. Mean fluorescent intensity (MFI) of UCP1 in the BAT (n = 8). C mRNA levels in the hypothalamus of C57BL6J mice fed a normal chow diet or HFD for 4 weeks (n = 8). D mRNA levels in the hypothalamus of FL and Pdgfrb^∆SYS-KO fed HFD for 4 weeks (n = 12–13). E Representative confocal images of Iba1 immunoreactivity (green) in the ARC and VMH of FL and Pdgfrb^∆SYS-KO fed HFD for 4 weeks (n = 8–12). Scale bar = 100 μm. Each region was indicated by a dotted line. The numbers of Iba1-positive microglia in the ARC and VMH were quantified. Data are presented as the mean ± SEM. *p < 0.05 and **p < 0.01