Fig. 3

HBPC-derived conditioned medium induces the inflammatory polarization of BMDMs. A Il6, Tnfa, and Nos2 mRNA levels in BMDMs treated with 100 ng/mL LPS and/or 100 ng/mL recombinant human PDGF-BB for 3 h (n = 4). B Upper; timeline of the experiment. Lower; Nos2 mRNA level in BMDMs (n = 7). HBPCs were treated with LPS and/or PDGF-BB for 1 h, and cells were washed and replaced with fresh medium. The conditioned medium (CM) of HBPCs was harvested after 24 h. BMDMs were then treated with each CM in serum-starved medium for 12 h and primed with 10 ng/mL LPS (stim. 1) for 24 h. C Upper; timeline of the experiment. Lower; Il6 and Tnfa mRNA levels in BMDMs (n = 5). BMDMs were treated with each CM in serum-starved medium for 12 h, primed with 10 ng/mL LPS (stim. 1) for 24 h, and stimulated with 100 ng/mL LPS (stim. 2). Data are presented as the mean ± SEM. *p < 0.05 and **p < 0.01, significantly different between each group. Stv starvation, stim. stimulation