Fig. 3

CRISPR-Cas9 Technique Knocking Out the blaNDM-1 Gene in CRKP Strains. A Structure of the plasmid vector PX458 (pSpCas9(BB)-2A-GFP). B Flow cytometry to detect transfection efficiency of the plasmid vector. C Fluorescence microscopy to detect transfection efficiency of the plasmid vector (upper: fluorescence microscope image; lower: light microscope image, scale bar: 100 μm). D PCR agarose gel electrophoresis to detect the silencing effect of the blaNDM-1 Gene (M: DNA molecular weight marker; 1, 2, 3: gene amplification products of CRKP strains; 4, 5, 6: gene amplification products of blaNDM-1-KO strains, N represents the number of deleted amino acids). E PCR block sequencing results. F RT-qPCR to detect the expression of the blaNDM-1 gene. G Western blot to detect the expression of NDM-1 protein. Quantitative data in the figure are represented as mean ± SD, with each experiment repeated 3 times. *P < 0.05 indicates significance compared to the CRKP group