Fig. 4
Effect of knocking out the blaNDM-1 gene on the virulence of CRKP strains. A Different strains' Minimum inhibitory concentrations (MICs) against IM, meropenem, ceftazidime, and amikacin determined by E-test. B Serum bactericidal experiment to assess the effect of silencing the blaNDM-1 gene on the cytotoxicity of CRKP strains. C Impact of different strains on the survival rate of Galleria mellonella larvae (n = 10). D Comparison of the number of white blood cells and neutrophils in mouse blood 7 days after infection with different strains (n = 5). E Evaluation of the impact of different strains on mouse survival rate (n = 10). F ELISA to detect the expression of inflammatory factors CRP, PCT, IL-1β, and IL-6 in mouse plasma 7 days after infection with different strains (n = 5). G Enumeration of bacteria in mouse lung tissue 7 days after infection with different strains (n = 5). H H&E Staining of mouse lung tissue 7 days after infection with different strains (scale bar: 100 μm, n = 5). Quantitative data in the figure are represented as mean ± SD, with each experiment repeated 3 times. *P < 0.05 indicates significance compared to the CRKP group; # indicates significance compared to the Normal group, #P < 0.05