Skip to main content
Fig. 3 | Molecular Medicine

Fig. 3

From: USF1 transcriptionally activates USP14 to drive atherosclerosis by promoting EndMT through NLRC5/Smad2/3 axis

Fig. 3

Ox-LDL-stimulated EndMT was restrained by USF1 down-regulation in HUVECs. sh-USF1-transfected HUVECs were treated with 100 µg/mL ox-LDL for 24 h. (A) USF1 mRNA level was evaluated by RT-qPCR. (B) Western blotting analysis of USF1, CD31, VE-Cadherin, α-SMA and vimentin levels in HUVECs. (C) Immunofluorescence staining of CD31, VE-Cadherin, α-SMA and vimentin in HUVECs (scale bar = 25 μm). (D) IL-6 and TNF-α production from HUVECs was assessed by ELISA. (E)&(F) Migration of HUVECs was assessed by transwell and scratch assays. n = 3. *p < 0.05, **p < 0.01, ***p < 0.001

Back to article page

Molecular Medicine

ISSN: 1528-3658