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Fig. 5 | Molecular Medicine

Fig. 5

From: USF1 transcriptionally activates USP14 to drive atherosclerosis by promoting EndMT through NLRC5/Smad2/3 axis

Fig. 5

USF1 promoted EndMT through modulation of USP14. The HUVECs were transfected with sh-USF1 together with or without oe-USP14, followed by stimulation with 100 µg/mL ox-LDL for 24 h. (A)&(B) USP14 level was assessed by RT-qPCR and Western blotting. (C) Western blotting analysis of protein levels of USF1, USP14, EndMT markers CD31, VE-Cadherin, α-SMA, and vimentin. (D) Immunofluorescence staining of CD31, VE-Cadherin, α-SMA, and vimentin in HUVECs (scale bar = 25 μm). (E) Release of IL-6 and TNF-α from HUVECs was evaluated by ELISA kits. (F)&(G) Migration capacity of HUVECs was determined by transwell and scratch assays. n = 3. *p < 0.05, **p < 0.01, ***p < 0.001

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Molecular Medicine

ISSN: 1528-3658