Fig. 5

ISO attenuates RANKL-induced F-actin ring formation and inhibited the expression of osteoclast marker genes. A Representative fluorescence images of actin stained RAW264.7 cells stimulated with RANKL without or with different concentrations of ISO (20, 40, 60, 100 μM). The actin cytoskeleton was stained red, and the nucleus was stained blue. B, C Quantification analyses of the average cell size (based on the cell spreading area of the actin ring) and number of nuclei per osteoclast. D Quantitative analysis of osteoclast marker gene expression after treatment with different concentrations of ISO, was determined using qRT-PCR. Values were presented as mean ± standard deviation (n = 3); Scale bar = 100 μm; *p < 0.05, **p < 0.01