Table 1 Primer sequences used for qRT-PCR analysis
Gene name | Primer sequence (5′–3′) forward | Primer sequence (5′–3′) reverse |
|---|---|---|
Cathepsin K | 5′- TATGACCACTGCCTTCCAATAC-3′ | 5′- GCCGTGGCGTTATACATACA-3′ |
TRAP | 5′- GGAGGCTTCGATGGTGTTT- 3′ | 5′- CTTTCCGGTCCCAAGGATTAG-3′ |
MMP-9 | 5′- CTGGAACTCACACGACATCTT-3′ | 5′- TCCACCTTGTTCACCTCATTT-3′ |
c-Fos | 5′- CGACCATGATGTTCTCGGGT -3′ | 5ʹ- TCGGCTGGGGAATGGTAGTA -3ʹ |
NFATc1 | 5ʹ-AGGACCCGGAGTTCGACTT -3ʹ | 5ʹ- AGGTGACACTAGGGGACACA -3ʹ |
GAPDH | 5′- AACAGCAACTCCCACTCTTC -3′ | 5′- CCTGTTGCTGTAGCCGTATT -3′ |
β-actin | 5′- AGGCCAACCGTGAAAAGATG -3′ | 5′- TGGCGTGAGGGAGAGCATAG-3′ |
HPRT1 | 5′- CAAACTTTGCTTTCCCTGGTT -3′ | 5′- TGGCCTGTATCCAACACTTC -3′ |