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Fig. 3 | Molecular Medicine

Fig. 3

From: WSB1, as an E3 ligase, restrains myocardial ischemia–reperfusion injury by activating β-catenin signaling via promoting GSK3β ubiquitination

Fig. 3

WSB1 inhibited IR-induced cell apoptosis in myocardial tissues. A CaV3 and EBD staining revealed the live and dead cells in ischemic penumbra area of rats with IR or/and WSB1 overexpression (CaV3 (green) indicated live cells and EBD (red) indicated dead cells, the scale bar represented as 50 μm). B TUNEL staining showed the apoptotic cells in penumbra area (the scale bar represented as 50 μm). C The levels of several apoptosis-related molecules, cleaved caspase-3, cleaved caspase-9, Bax and Bcl-2 were determined by western blot in penumbra area. D The levels of Wnt1, total β-catenin, non phosphor (active) β-catenin and nuclear β-catenin in penumbra area. E The mRNA levels of β-catenin targets, c-MYC and cyclin D1 in penumbra area. (IR, ischemia–reperfusion; WSB1, WD repeat and SOCS box containing 1; CaV3, Caveolin-3; EBD, Evans blue dye; ***p < 0.001)

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Molecular Medicine

ISSN: 1528-3658