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Fig. 4 | Molecular Medicine

Fig. 4

From: WSB1, as an E3 ligase, restrains myocardial ischemia–reperfusion injury by activating β-catenin signaling via promoting GSK3β ubiquitination

Fig. 4

WSB1 ameliorated OGD/R-caused apoptosis in myocardial cells. The mRNA A and protein levels of WSB1 B in myocardial cells with OGD/R treatment and WSB1 overexpression/knockdown were assessed with real-time PCR and western blot. C CCK-8 assay was used to measure the viability of myocardial cells. D and E Flow cytometry was performed to evaluate the apoptosis of myocardial cells. F The levels of apoptosis-related molecules, cleaved caspase-3, cleaved caspase-9, Bax and Bcl-2 were detected by western blot in myocardial cells with OGD/R treatment and ectopic expression or silencing of WSB1. (OGD, oxygen-sugar deprivation; WSB1, WD repeat and SOCS box containing 1; PI, propidium iodide; *p < 0.05, **p < 0.01, ***p < 0.001)

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Molecular Medicine

ISSN: 1528-3658