Fig. 4
From: Huntingtin HTT1a is generated in a CAG repeat-length-dependent manner in human tissues
HTT1a is generated in a CAG repeat-length-dependent manner in lymphoblastoid cell lines
AHTT1a and HTT exon 2 (HTTex2) expression in human lymphoblastoid cell lines was analysed in lines with a CAG repeat in the control (control), adult-onset (HD) or juvenile-onset range (HD juvenile). The black line represents the linear model of expression level with CAG repeat length calculated from the mean of three different time points in culture ± SEM (see also Figure S1B). R2 (coefficient of determination) and P value for the fit of the linear modelling are shown. Grey areas represent the standard error of the regression model. B Exemplary CAG repeat size traces from lymphoblastoid lines analysed in A. CAG repeat length of the longer allele determined from total blood DNA is given in brackets. CHTT1a and HTT exon 2 expression analysis of CD123+/CD19+ FACS sorted cells (dotted lines) versus the total population (solid lines). Linear modelling was as in A. D Exemplary CAG repeat size traces from lymphoblastoid lines analysed in C. CAG repeat length of the longer allele determined from total blood DNA is given in brackets.