Table 1 Assay sequences
From: Huntingtin HTT1a is generated in a CAG repeat-length-dependent manner in human tissues
Assay | Name | Sequence (5’-3’) | Modification |
|---|---|---|---|
RT-reaction | UAPdT18 | GGCCACGCGTCGACTAGTACTTTTTTTTTTTTTTTTTT | |
HTT exon 2 | HTTex2_f | AAAGAAAGAACTTTCAGCTACCAAGAA | |
HTTex2_r | CTGACAGACTGTGCCACTATG | ||
HTTex2_p | TCACATATTGTCAGACAATGATTCACACGGTCT | 5’-HEX, 3’-BHQ2 | |
HTT1a | HTT1a_f | GGATCCACACTCAAAACATTTA | |
UAP18qPCR | CACGCGTCGACTAGTAC | ||
HTT1a_p | TCTTATTCAGACAACAAGGAGGAAAAATAAAATACC | 5’-FAM, 3’-BHQ1 |