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Fig. 1 | Molecular Medicine

Fig. 1

From: Iguratimod, an allosteric inhibitor of macrophage migration inhibitory factor (MIF), prevents mortality and oxidative stress in a murine model of acetaminophen overdose

Fig. 1

Kinetic analysis of MIF-T-614 complex. Chemical structures of (A) the MIF inhibitor T-614 and (B) the substrate 4-HPP for MIF-induced keto-enol tautomerization. (C) The Michaelis-Menten and (D) Lineweaver-Burk plots demonstrate that the inhibitor binds MIF in a non-competitive fashion. The inhibition potency of T-614 against MIF’s keto-enol tautomerase activity was investigated at concentrations ranging from 0–50 µM and yielded an inhibition constant (Ki) of 16 µM. Data is expressed as mean ± SD from experiments done in triplicate (n = 3). T614 = iguratimod

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Molecular Medicine

ISSN: 1528-3658