Fig. 1
From: Hypothalamic POMC neuron-specific knockout of MC4R affects insulin sensitivity by regulating Kir2.1
Ablation of MC4R in the hypothalamus leads to obesity. (A) Immunoflurescent staining for POMC and MC4R of the hypothalamus of POMC-MC4Rflox/flox mice. Scale bar = 500 μm above the picture, Scale bar = 200 μm below the picture. (B) Body-weight in MC4R WT and KO mice fed a chow or HFD from 8 wk of age. (C) Food intake assessed during the first 13 wk of diet treatment. (D) Fat mass at 21 wk old. Data are presented as means ± SD. *P < 0.05, **P < 0.01, two-tailed Student’s t test was used for comparison for (A) MC4R-positive POMC cells; two-way ANOVA for (D) fat volume. Repeated measures ANOVA for multiple comparisons for (B) body weight and (C) food intake