Fig. 3
From: Hypothalamic POMC neuron-specific knockout of MC4R affects insulin sensitivity by regulating Kir2.1
Ablation of MC4R in the hypothalamus decreased hepatic insulin sensitivity. (A and B) GTT (A) and the AUC of GTT (B) of 21-wk-old mice. (C and D) ITT (C) and the AUC of ITT (D) of 21-wk-old mice. (E, F and G) Relative protein expressions of IR and Akt were analyzed by western blot in BAT. (H, I and J) Relative protein expressions of IR and Akt were analyzed by western blot in liver. (K, L and M) Relative protein expressions of IR and Akt were analyzed by western blot in soleus. Two-way ANOVA for (B) AUC of GTT, (D) AUC of ITT, (F, I, L) p-IR/IR protein, and (G, J, M) p-AKT/AKT. Repeated measures ANOVA for multiple comparisons for (A) glucose level (GTT) and (C) glucose level (ITT). *P < 0.05 vs. chow-Control; #P < 0.05 vs. HFD-Control.