Fig. 4
From: Hypothalamic POMC neuron-specific knockout of MC4R affects insulin sensitivity by regulating Kir2.1
Ablation of MC4R drives activation of POMC neurons. (A and B) POMC western blot and densitometric quantification in hypothalamus. (C and D) Immunofluorescence for POMC neurons (red), c-Fos (green) and merge in ARC sections and integrated density quantification in POMC neurons and colocalization in 21-weeks old control and MC4R mice treated with or without HFD. Scale bar = 100 μm. Two-way ANOVA. *P < 0.05 vs. chow-Control; #P < 0.05 vs. HFD-Control.