Fig. 5
From: Hypothalamic POMC neuron-specific knockout of MC4R affects insulin sensitivity by regulating Kir2.1
MC4R negatively regulates the expression of Kir2.1. (A) Transcriptome analysis found that the expression of potassium inwardly-rectifying channel subfamily J member 2, KCNJ2, or Kir2.1 was down-regulated. (B) Immunofluorescence for Kir2.1 (green) and merge. Scale bar = 20 μm. (C) Immunofluorescence for Kir2.1 (green), MC4R (purple) and POMC (red) in HFD mice. Scale bar = 200 μm at the top, Scale bar = 100 μm at the bottom. (D-F) Primary hypothalamic neurons were transfected with MC4R shRNA plasmid, and the transfection efficiency and the protein expression of Kir2.1 were analyzed by western blot. (G) Co-IP assay detected the interaction of MC4R and Kir2.1. *P < 0.05, **P < 0.01. One-way ANOVA with Dunnett's post hoc test for (D) relative MC4R protein expression and (E) relative protein expression of Kir2.1. Two-way ANOVA for (B) % Kir2.1 positive of ARC.