Fig. 1

Elder Itln-1 knockout mice exhibit spontaneous BPH. A Relative gene expression levels of Itln-1 in the small intestine of 5-month-old wild type (WT) mice and Itln-1^−/− mice. n = 4, t-test. B Gross anatomy of prostate tissues from 5-month-old WT and Itln-1^−/− mice. C–F The body weight (C), prostate weight (D), prostate index (E), and serum levels of PAP (F) in 5-month-old (5 M) or 10-month-old (10 M) WT and Itln-1^−/− mice, respectively. n = 6 for 5-month-old mice, n = 4 for 10-month-old mice, two-way ANOVA. The prostate index was calculated as the ratio of prostate wet weight (mg) to the body weight (g) of the mice. G Representative images of H&E staining of prostate tissues from WT and Itln-1^−/− mice, observed under 200-fold magnification. Scale bar: 50 μm. H, I Area of lumens (H) and stromal/epithelial ratio (I) of WT and Itln-1^−/− mice, respectively. n = 6 for 5-month-old mice, n = 4 for 10-month-old mice, two-way ANOVA. The values of the individual statistical significances and statistical comparisons were indicated in the figure