Fig. 3
From: Omentin-1 inhibits the development of benign prostatic hyperplasia by attenuating local inflammation
Omentin-1 suppresses TNF-α-activated macrophage-mediated proliferation of BPH-1 cells. A The effect of ITLN-1 protein on BPH-1 cell viability, as measured by CCK8 assay. n = 5, t-test. B Cell viability of BPH-1 cells after indicated different treatments. n = 3, one-way ANOVA. C, D Representative images of EdU staining (C) and percentage of EdU positive nuclei in per field (D) of BPH-1 cells after indicated different treatments, observed under 100-fold magnification. Scale bar: 100 μm. Data represented as mean ± SEM. n = 3, one-way ANOVA. E–G The concentration of TNF-α (E), IL-2 (F), and IL-6 (G) in the concentrated supernatant of RAW264.7 macrophages stimulated with Vehicle, TNF-α, ITLN-1, or TNF-α + ITLN-1. n = 3, one-way ANOVA. The values of the individual statistical significances and statistical comparisons were indicated in the figure