Skip to main content
Fig. 1 | Molecular Medicine

Fig. 1

From: Vascular smooth muscle cells in response to cholesterol crystals modulates inflammatory cytokines release and promotes neutrophil extracellular trap formation

Fig. 1

Localization of VSMCs and CC in human atherosclerotic plaque. A Immunohistochemistry was performed on paraffin embedded sectioned plaques which were stained for SMA and CD68 positive cells. B Confocal microscopy was performed to visualize CC uptake in VSMCs at 8 h and 16 h (CC shown in white by polarized light, Actin in red by Rhodamine phalloidin, and nucleus in blue by DAPI staining), Scale bar, 250 μm. C Z stacking 3D image showing CC, nucleus and actin filament (Yellow arrows) in the same plane confirming the uptake. Scale bar, 100 μm, Objective 20X. D Dose dependent uptake of CC in VSMCs for 24 h. Data are representative of experiments from VSMCs of 4 donors and displayed as mean ± SD. *p < 0.05, **p < 0.01

Back to article page

Molecular Medicine

ISSN: 1528-3658