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Fig. 4 | Molecular Medicine

Fig. 4

From: Vascular smooth muscle cells in response to cholesterol crystals modulates inflammatory cytokines release and promotes neutrophil extracellular trap formation

Fig. 4

Neutrophil ROS production and NETs formation in response to conditioned medium from CC treated VSMCs. PMN were treated with CC, conditioned medium from unstimulated (Control CM) and CC stimulated (CC CM) VSMCs and. Viability of PMN after 4 h (A) and 24 h (B) of treatments. Phagocytosis of pHrodoTM Red after 1 h (C) and CC after 1 h (D) in response to the treatments for 1 h in PMN. ROS was measured in response to the treatments at 90 min (E). Data are representative of samples from 3 different experiments and displayed as mean ± SD * p < 0.05, ** p < 0.01, *** p < 0.001. NETs formation in unstimulated PMN, CC treated PMN, conditioned medium (CM) from unstimulated VSMCs, conditioned medium from CC treated VSMCs and PMA for 3 h (F). Scale bar 250 μm, objective 10X; Scale bar 250 μm, objective 20X; Scale bar 100 μm, objective 40X

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Molecular Medicine

ISSN: 1528-3658