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Fig. 5 | Molecular Medicine

Fig. 5

From: Vascular smooth muscle cells in response to cholesterol crystals modulates inflammatory cytokines release and promotes neutrophil extracellular trap formation

Fig. 5

IL33 expression in response to CC in VSMCs. A VSMCs were treated with CC for 24 h and IL33 release was measured using ELISA. B VSMCs were treated with CC for 24 h that was preincubated with PI3K inhibitor for 1 h. IL33 release was measured using ELISA. C Uniform manifold approximation and projection (UMAP) visualization of IL33 and IL1RL1 gene expression in different cell types of carotid plaque from Slenders et al. (n = 38). D Representative image showing calecin AB labelled neutrophil adhesion on endothelial cells in response to IL33 alone, conditioned medium (CM) from VSMC treated with and without CC (0.5 mg/ml) for 24 h, Scale bar, 100 μm, objective 4X. E Quantification of neutrophil adhesion on endothelial cells in response to treatment. Data are representative of experiments from VSMCs of 4 donors and displayed as mean ± SD.* p < 0.05, *** p < 0.001

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Molecular Medicine

ISSN: 1528-3658