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Fig. 6 | Molecular Medicine

Fig. 6

From: Vascular smooth muscle cells in response to cholesterol crystals modulates inflammatory cytokines release and promotes neutrophil extracellular trap formation

Fig. 6

Blocking IL33 in the conditioned medium from VSMCs. A PMN were treated with 100 ng/ml of recombinant IL-33 that were pre-incubated with 1ug/ml of Anti-hIL-33-IgG or DPI for 3 h. B Conditioned medium from CC treated VSMCs were pre-incubated with 1ug/ml of Anti-hIL-33-IgG or DPI for 3 h. Relative fluorescence intensity of Nets (C, D). ROS production was measured in PMN in response to 100 ng/ml of recombinant IL-33 (E),conditioned medium from unstimulated (Control CM/CTL CM) (F) and CC treated (CC CM) VSMCs (G) that were pre-incubated with 1ug/ml of Anti-hIL-33-IgG for 30 min. Data are representative of samples from 3 different experiments and displayed as mean ± SD * p < 0.05, ** p < 0.01, **** p < 0.001

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Molecular Medicine

ISSN: 1528-3658