Fig. 4

Detection of the expression of Col-I and α-SMA in NIH-3T3 cells by immunofluorescence. (A). Immunofluorescence detection of Col-I (magnifcation×200, scale bars = 50 μm). DAPI (blue, nuclear staining), Col-I (red). Magnifcation×200, scale bars = 50 μm. (B). Fluorescence analysis of Col-I. (C). Immunofluorescence detection of α-SMA. (magnifcation×200, scale bars = 50 μm). DAPI (blue, nuclear staining), α-SMA (red). (D). Fluorescence analysis of α-SMA. (E). Protein expressions of Smad2 and P-Smad2 in NIH-3T3 cells. (F). Quantitative analysis of P-Smad2. ^###P < 0.001, ^####P < 0.0001 vs. Control; *P < 0.05,**P < 0.01,****P < 0.0001 vs. TGF-β1; ^ΔP < 0.05, ^ΔΔΔΔP < 0.0001, tLAP vs. SE-tLAP