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Fig. 3 | Molecular Medicine

Fig. 3

From: CHMP5 attenuates osteoarthritis via inhibiting chondrocyte apoptosis and extracellular matrix degradation: involvement of NF-κB pathway

Fig. 3

The identification of human chondrocytes and verification of transfection efficiency. (A) The chondrocytes were identified by detecting the levels of collagen-II using immunofluorescence (IF) assay. Bar: 50 μm. (B) The infection efficiency was detected by real-time PCR and western blot 24 h after the adenovirus infection. Adenovirus-mediated CHMP5 overexpression (Ad-CHMP5) increased the levels of CHMP5, and (C) CHMP5 small hairpin RNA (Ad-shCHMP5) decreased the levels of CHMP5 in the chondrocytes. ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658