Fig. 1

Fcgr2b is lowly expressed in ARDS rats. Notes A, HE staining to measure histological alterations in rat lung tissues; B, PAS staining to examine glycogen accumulation in rat lung tissues; C, Masson’s staining to evaluate the level of fibrous deposition in rat lung tissues; D, ELISA to detect the levels of inflammatory factors IL-17 and IL-6 in rat BALF; E-F, RNA-seq analysis of heat map and volcano map of differentially expressed genes in rat lung tissues; G-H, RT-qPCR and western blot to test Fcgr2b mRNA and protein expression in rat lung tissues. I, dual-labeled immunofluorescence to assess the fluorescence intensity of Fcgr2b and CD31 in lung tissue. Differences between two groups were compared using the t-test (A-B), and differences among multiple groups were compared using one-way or two-way analysis of variance with Tukey’s post-hoc test. ARDS, acute respiratory distress syndrome; BALF, bronchoalveolar lavage fluid