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Fig. 4 | Molecular Medicine

Fig. 4

From: Elk1 enhances inflammatory cell infiltration and exacerbates acute lung injury/acute respiratory distress syndrome by suppressing Fcgr2b transcription

Fig. 4

LPS-induced cell injury in PMVECs was restrained by overexpressing Fcgr2b. Notes A, RT-qPCR for Fcgr2b level in PMVECs; B, CCK-8 assay for the activity of PMVECs in each group; C, detection of LDH release in each group of PMVECs; D, TUNEL assay for the apoptosis of PMVECs in each group; E, tubule formation assay for the angiogenic capacity of PMVECs in each group; F, western blot for Fcgr2b, VE-cadherin, and β-catenin expression in each group of PMVECs. N = 3 ~ 5. Differences between two groups were compared using the t-test (A), and differences among multiple groups were compared using one-way (B-E) or two-way (F) analysis of variance with Tukey’s post-hoc test. LPS, lipopolysaccharide; PMVECs, pulmonary microvascular endothelial cells; LDH, lactate dehydrogenase

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Molecular Medicine

ISSN: 1528-3658