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Fig. 5 | Molecular Medicine

Fig. 5

From: Elk1 enhances inflammatory cell infiltration and exacerbates acute lung injury/acute respiratory distress syndrome by suppressing Fcgr2b transcription

Fig. 5

ARDS models have high Elk1 expression, which represses Fcgr2b transcription. Notes A, Immunohistochemistry to examine Elk1 expression in lung tissues of ARDS rats (n = 6/group); B-C, RT-qPCR and western blot to determine Elk1 expression in LPS-induced PMVECs (N = 3); D, RT-qPCR to detect Elk1 and Fcgr2b expression in PMVECs after lentiviral infection and LPS induction (N = 3); E, ChIP-qPCR to assess the binding relationship between Elk1/H3K9me3 and Fcgr2b promoter (N = 3); F, dual-luciferase reporter assay to evaluate the transcriptional regulation of Elk1 to Fcgr2b (N = 3). Differences between two groups were compared using the t-test (A-C, F), and differences among multiple groups were compared using one-way (E) or two-way (D) analysis of variance with Tukey’s post-hoc test. ARDS, acute respiratory distress syndrome; LPS, lipopolysaccharide; PMVECs, pulmonary microvascular endothelial cells; H3K9me3, histone 3 lysine 9 trimethylation

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Molecular Medicine

ISSN: 1528-3658