Fig. 1

Bleomycin causes cellular senescence in AECs. A, B A549 and MLE-12 cells were treated with different concentrations of bleomycin for 3 days. SA‐β‐gal staining was performed to detect cellular senescence (magnification ×100). C, D The expression of senescence-related markers p21^WAF1 and p16^ink4a in A549 and MLE-12 cells was analyzed by Western blotting. E, F The relative mRNA expression of SASP factors (IL-1α, IL-1β, IL-8, and CXCL-1) in A549 and MLE-12 cells was analyzed by RT-PCR. G EdU incorporation assay was performed to detect A549 cell proliferation and the EdU-positive cells were quantitated. Significance markers: *p < 0.05; **p < 0.01; ***p < 0.001 compared to control; n = 3