Fig. 2

Bleomycin treatment induces sustained DNA damage. A A549 cells were exposed to bleomycin at a dose of 10 μM, followed by immunostaining for γH2AX foci (red) at indicated time points. B A549 cells were treated with bleomycin (10 μM) for the indicated time points, and then a comet assay was performed using neutral electrophoresis. C Cell cycle distributions were examined by flow cytometry after 1, 2, and 3 days of bleomycin exposure (10 μM). Significance markers: *p < 0.05; **p < 0.01 compared to control; n = 3