Fig. 5

Silencing of Rad51 enhances bleomycin-induced cellular senescence in AECs. A Western blotting of the Rad51 expression in A549 cells transfected with control or Rad51 siRNA. B A549 cells were transfected with control or Rad51 siRNA for the indicated time, followed by SA-β-gal staining (magnification ×100). C, D After transfection with control or Rad51 siRNA, A549 cells were incubated with bleomycin for 72 h. SA‐β‐gal staining was performed to detect cell senescence (magnification ×100). E Western blotting of p21^WAF1 and p16^ink4a expression in bleomycin-treated A549 cells with control or Rad51 siRNA transfected. F RT-PCR analysis of SASP factors (IL-1α, IL-1β, IL-8, and CXCL-1) in bleomycin-treated A549 cells with control or Rad51 siRNA transfected. Significance markers: *p < 0.05 compared to control; n = 3