Fig. 6

Overexpression of Rad51 eliminates bleomycin-induced inhibition of DNA repair and senescence in AECs. A Western blotting of the Rad51 expression in A549 cells infected with Rad51 expression vector. B Cells were transfected with control vector or Rad51 expression vector, and further treated with bleomycin (10 μM) for 24, 48, or 72 h. The expression of γH2AX was measured by Western blotting. C, D After transfection with control vector or Rad51 expression vector, A549 cells were incubated with bleomycin for 72 h. SA‐β‐gal staining was performed to detect cell senescence (magnification ×100). E Western blotting of p21^WAF1 and p16^ink4a expression in bleomycin-treated A549 cells with control or Rad51 vectors transfected. F RT-PCR analysis of SASP factors (IL-1α, IL-1β, IL-8, and CXCL-1) in bleomycin-treated A549 cells with control or Rad51 vectors transfected. Significance markers: *p < 0.05; **p < 0.01 compared to control; n = 3