Table 1 The primer sequences used for RT-PCR
From: Bleomycin induces senescence and repression of DNA repair via downregulation of Rad51
Gene | Direction | Primer Sequence (5â˛-3â˛) |
|---|---|---|
Human | ||
 CXCL1 | Forward | TCCTGCATCCCCCATAGTTA |
Reverse | CTTCAGGAACAGCCACCAGT | |
 IL-1ι | Forward | GGTTGAGTTTAAGCCAATCCA |
Reverse | TGCTGACCTAGGCTTGATGA | |
 IL-1β | Forward | CTGTCCTGCGTGTTGAAAGA |
Reverse | TTGGGTAATTTTTGGGATCTACA | |
 IL8 | Forward | AGACAGCAGAGCACACAAGC |
Reverse | ATGGTTCCTTCCGGTGGT | |
 Rad51 | Forward | GGTGAAGGAAAGGCCATGTA |
Reverse | GGGTCTGGTGGTCTGTGTT | |
 GAPDH | Forward | CATGAGAAGTATGACAACAGCCT |
Reverse | AGTCCTTCCACGATACCAAAGT | |
Mouse | ||
 CXCL1 | Forward | GACCATGGCTGGGATTCACC |
Reverse | CGCGACCATTCTTGAGTGTG | |
 IL-1ι | Forward | CCCATGATCTGGAAGAGACCA |
Reverse | CAAACTTCTGCCTGACGAGC | |
 IL-1β | Forward | ATGCCACCTTTTGACAGTGATG |
Reverse | TGATGTGCTGCTGCGAGATT | |
 IL8 | Forward | CCTGATGCTCCATGGGTGAA |
Reverse | ACAGAAGCTTCATTGCCGGT | |
 GAPDH | Forward | CAGGAGAGTGTTTCCTCGTCC |
Reverse | TGAGGTCAATGAAGGGGTCG | |