Figure 3
From: Requirement of STAT3 Activation for Differentiation of Mucosal Stratified Squamous Epithelium
Activated STAT3 is enhanced by culturing in suspension. A: 40 of whole cell extracts were analyzed by Western blot, using anti-phospho-STAT3 or anti-STAT3 as indicated in Materials and Methods. B: The ratio of phosphorylated STAT3/total STAT3 after 24 and 48 h in suspension culture showed significant differences from 0 h (*P < 0.01). Data are means ± SD from 2 independent duplicate experiments. C: To confirm that STAT3 phosphorylation was indicative of activity, normal cells were transfected with a STAT3-responsive luciferase construct prior to suspension culture.