Figure 4
From: Requirement of STAT3 Activation for Differentiation of Mucosal Stratified Squamous Epithelium
Dominant-negative and constitutive active STAT3 constructs are functional in suspension cultures. Effects of STAT3C or Y705F expression on STAT3-dependent transcription were measured by luciferase assay. A: STAT3-driven luciferase activity was greatly increased in STAT3C-transfected cells, but not in Y705F-transfected cells. B: Transfection of STAT3 mutant constructs (STAT3C or Y705F) into normal laryngeal cells did not alter survival as measured by the XTT assay. Survival of cells at 0 h in each group was set to 1. Data are means ± SD from triplicate data.