Figure 6
From: Requirement of STAT3 Activation for Differentiation of Mucosal Stratified Squamous Epithelium
STAT3 activity is required for differentiation. Cells were cultured in suspension 24 h post-transfection. At indicated times, keratin extracts were prepared and analyzed by Western blot. Expression of a dominantnegative STAT3 (Y705F) construct inhibited K13 expression. Fast green staining shows equal loading of protein. Relative ratio of K13/K14 shows that STAT3 (Y705F) significantly inhibited differentiation compared with the control (*P = 0.02). Data are means ± SD from 2 independent duplicate experiments.