Sampling-Dependent Up-regulation of Gene Expression in Sequential Samples of Human Airway Epithelial Cells

Table 2 Genes up-regulatedin the airway epithelium of the 2nd sampling site relative to the first sampling site ^a

Category^b	Gene ID^c	Description^d	P value^e	Average left/right ratio in right-first samples^f	Average right/left ratio in left-first samples^g
Transcription factors	J04076	Early growth response 2	0.01005	6.94	18.00
	X63741	Early growth response 3	0.00522	5.72	1.63
	V01512	fos	0.00031	4.46	4.88
	U20734	jun B	0.00972	3.26	3.02
	X52541	Early growth response 1	0.00020	2.52	2.90
	U70663	Kruppel-like factor 4	0.00167	2.32	2.08
	S77154	Nuclear receptor subfamily 4, group A, member 2	0.00523	2.30	2.57
	U09851	Zinc finger protein 148 (pHZ-52)	0.00626	2.26	0.99
	D15050	Transcription factor 8	0.00252	2.25	2.75
	L19314	Hairy homolog (Drosophila)	0.00266	2.04	1.98
Signal transduction	L11329	Dual specificity phosphatase 2	0.00179	6.17	6.32
	U15932	Dual specificity phosphatase 5	0.00647	3.51	3.19
	J00277	H-ras	0.00618	2.21	1.54
	X68277	Dual specificity phosphatase 1	0.00026	2.16	2.20
	U10550	GTP-binding protein overexpressed in skeletal muscle	0.00566	2.04	1.64
Cell cycle/growth	U22398	Cyclin-dependent kinase inhibitor 1C (p57, Kip2)	0.00630	2.28	0.82
	Y09943	BTG family, member 2	0.00359	2.26	5.41
	X62048	WEE1 + homolog p ](Schizosaccharomyces pombe)	0.00440	2.00	2.58
Apoptosis	S81914	Immediate early response 3	0.00035	2.15	1.21
	D90070	PMA-induced protein 1	0.00234	2.53	4.35
Other	X16699	Cytochrome P450, subfamily IVB, polypeptide 1	0.00276	2.66	2.14
	HG2724-HT2820	TLS CHOP fusion oncogene	0.00421	2.41	2.21
	M92843	Zinc finger protein 36, C3Htype	0.00335	2.02	3.05

^aThe genes listed are the 23 genes that were expressed, on average, at more than 2-fold higher level in the airway epithelium of the left lung compared with the right lung of each individual, using 20 individuals whose right lung was sampled 1st (P < 0.01 by paired f-test).
^bCategories assigned are based on GenBank, PubMed, and literature searches.
^cGenBank or TIGR unique identifier.
^dDescription is based on GenBank identifier together with PubMed, Locus Link, GenBank, and OMIM searches.
^eP value was calculated based on 20 individuals (40 microarrays) whose right lungs were sampled 1st, using the paired Student t-test
^fThe average ratio for the 20 individuals whose right lungs were sampled 1st was assessed as follows: the ratio of left airway epithelium to right airway epithelium expression levels for all genes labeled “P” in at least 1 microarray were calculated for each individual, and then the average of all individual ratios for each gene was determined. ^gThe average ratio for the 4 individuals whose left lungs were sampled 1st was assessed as follows: the ratio of left airway epithelium to right airway epithelium expression levels for all genes labeled “P” in at least 1 microarray were calculated for each individual, and then the average of all individual ratios for each gene was determined.

ISSN: 1528-3658