Figure 6
From: Disease-Associated Prion Protein Elicits Immunoglobulin M Responses In Vivo
Isotype profile of anti-PrP polyclonal responses. FVB-N Prn-Po/o mice were immunized with either soluble or bead-bound recombinant a or β PrP91–231 (panel A), PrPSc-Dynabeads (panel B), or PrPc-Dynabeads (panel C), and the isotype of anti-PrP produced was determined using Ig subclass-specific secondary antibodies in the standard recombinant PrP ELISA (see Materials and Methods). Data points represent the mean serum OD values of 1.24 ± 0.41, derived from 5 mice immunized with each antigen. Sera from unimmunized mice or mice similarly immunized with CFA/IFA emulsified with PBS gave OD values < 0.05. Representative of 2 experiments.